Figure 1

Phenotypes of bcl3^−/− zebrafish. (A) Representative images of bcl3^+/+, bcl3^+/−, and bcl3^−/− zebrafish at 4 months old. (B) Body weight, (C) body length, (D) body surface area (BSA), and (E) body mass index (BMI) of bcl3^+/+, bcl3^+/−, and bcl3^−/− zebrafish. BSA was defined by: BSA (cm²) = 8.46 × (body weight (g))^0.66; BMI was calculated as body weight (g) divided by body length square (cm²). Body length data were normally distributed and analyzed using one-way ANOVA followed by Tukey’s post hoc test; whereas body weight data, BSA, and BMI values did not meet normality assumptions and were analyzed using the Kruskal–Wallis test with Holm-corrected Mann–Whitney U post hoc comparisons. (F) Genotype ratio of bcl3^+/+, bcl3^+/−, and bcl3^−/− larvae at indicated dpf. At each time point, 48 larvae were genotyped. (G) Survival curves of zebrafish WT and bcl3^−/− from 35 to 150 dpf, analyzed using the log-rank (Mantel–Cox) test. *** p < 0.001, ** p < 0.01, ns indicates not significant. Error bars represent standard deviation (SD).