FIGURE

Figure 4.

ID

ZDB-FIG-251130-27

Publication

Ren et al., 2025 - RPGRORF15 Mutations Disrupt Lysosomal Lipid Metabolism in Retinal Pigment Epithelium Cells and Cause Retinitis Pigmentosa

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Figure 4.

Loss of rpgra alters phagocytosis-related pathways and protein levels in zebrafish RPE cells. (A) Volcano plot depicting differentially expressed genes in rpgra^−/− zebrafish compared with WT. (B) The differentially expressed genes from top 50 KEGG terms were classified into the 6-enrichment categories. (C) KEGG pathways enrichment analysis based on differentially expressed genes. (D) TEM images of retinal ultrathin sections from WT and rpgra^−/− zebrafish at 2 mpf. AL, abnormal lysosomes; PL, undegraded phagolysosomes; L, lysosomes. Scale bar = 3 µm. (E) Detection and quantification (F) of the protein level of phagocytosis related-gene in zebrafish RPE cells by Western blot at 1.5 mpf and 2 mpf (n = 3 biological replicates). Data are presented as mean ± SD. Statistical significance is indicated as not significant (ns), *P < 0.05, **P < 0.01, ***P < 0.001.

Expression Data

Antibodies:	Ab2-lamp2 Ab2-mertk Ab2-rab7 Ab3-ctsb Ab8-lamp1
Fish:	WT rpgra^{zf4007/zf4007}
Anatomical Term:	retinal pigmented epithelium
Stage:	Days 45-89

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rpgra^{zf4007/zf4007}
Observed In:	retinal pigmented epithelium retinal pigmented epithelium lipid droplet retinal pigmented epithelium lysosome retinal pigmented epithelium lysosome organization retinal pigmented epithelium phagolysosome
Stage:	Days 45-89

Phenotype Detail

Acknowledgments

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