FIGURE

Figure 2.

ID

ZDB-FIG-251130-25

Publication

Ren et al., 2025 - RPGRORF15 Mutations Disrupt Lysosomal Lipid Metabolism in Retinal Pigment Epithelium Cells and Cause Retinitis Pigmentosa

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Figure 2.

There is lipid accumulation in RPE cells of rpgra^−/− zebrafish. (A) Ultrastructural analysis of the retinal RPE layer from 2 and 4 mpf zebrafish. Scale bar = 5 µm. (B) Nile red staining in frozen retinal sections of 1.5, 2, and 4 mpf zebrafish. Scale bar = 30 µm. (C) Nile red staining of RPE flat mounted eyecups from 1.5, 2, and 4 mpf zebrafish. White arrows indicate lipid droplet (LD). Scale bar = 20 µm. (D) Diameter of LD size was categorized into three groups: black (>3 µm), dark grey (1–3 µm), and grey (0–1 µm). (E) TG concentrations were measured in WT and rpgra^−/− zebrafish (n = 3 biological replicates). Data are presented as mean ± SD. Statistical significance is indicated as ns (not significant), *P < 0.05, **P < 0.01, ***P < 0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rpgra^{zf4007/zf4007}
Observed In:	retinal pigmented epithelium lipid droplet retinal pigmented epithelium triglyceride
Stage Range:	Days 45-89 to Adult

Phenotype Detail

Acknowledgments

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