ZFIN Image: Ren et al., 2025, Figure 4.

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Figure Caption

Figure 4.

Loss of rpgra alters phagocytosis-related pathways and protein levels in zebrafish RPE cells. (A) Volcano plot depicting differentially expressed genes in rpgra^−/− zebrafish compared with WT. (B) The differentially expressed genes from top 50 KEGG terms were classified into the 6-enrichment categories. (C) KEGG pathways enrichment analysis based on differentially expressed genes. (D) TEM images of retinal ultrathin sections from WT and rpgra^−/− zebrafish at 2 mpf. AL, abnormal lysosomes; PL, undegraded phagolysosomes; L, lysosomes. Scale bar = 3 µm. (E) Detection and quantification (F) of the protein level of phagocytosis related-gene in zebrafish RPE cells by Western blot at 1.5 mpf and 2 mpf (n = 3 biological replicates). Data are presented as mean ± SD. Statistical significance is indicated as not significant (ns), *P < 0.05, **P < 0.01, ***P < 0.001.

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Acknowledgments

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