Figure 3.

Temporal resolution of flicker ERG responses is reduced in eaat2b mutant fish. (A) Representative flicker ERG responses to a 5 Hz stimulus in WT controls (black) and eaat2b^−/− larvae (red). Corresponding FFT power plots for WT (A′) and eaat2b^−/− (A″) were generated in MATLAB from the traces shown in (A). (B) Representative flicker ERG responses to an 8 Hz stimulus in WT controls (black) and eaat2b^−/− larvae (red). Corresponding FFT power plots for WT (B′) and eaat2b^−/− (B″) were generated from the traces shown in (B). (C) Normalized power of flicker ERG responses at specified frequencies in WT controls and eaat2b^−/− larvae. Power values were normalized to the 50 Hz line-noise peak. Statistical significance: p = 0.0039 at 5 Hz, p = 0.0007 at 8 Hz, p = 0.0031 at 10 Hz, p = 0.0133 at 15 Hz. Statistical analysis was performed using t-tests and nonparametric tests in GraphPad Prism, v. 8. Data are presented as mean ± s.e.m. Significance levels are indicated as follows: *p < 0.05; **p < 0.01; ***p < 0.001.