Fig. 3

Overexpression of miR-130b-3p reduces osteogenic ability in primary osteoblasts from non-AIS control patients.a. RNA expression level of miR-130b in the overexpression group is elevated (n = 3,) b. OCN mRNA expression level is reduced in the miR-130b overexpression group (n = 8) c. RUNX2 expression level is reduced in the miR-130b overexpression group (n = 3) d. OPN mRNA expression level is reduced in the miR-130b overexpression group (n = 3) e. Western blot (WB) analysis showing osteogenic marker expression after miR-130b overexpression f. RUNX2 protein expression level is reduced in the miR-130b overexpression group (n = 3) g. OPN protein expression level is reduced in the miR-130b overexpression group (n = 3) h. Immunofluorescence staining of OCN in osteoblasts from the control group (top) and the miR-130b-3p mimic group (bottom) i. Alizarin red s staining after 14 days of osteogenic differentiation in the miR-130b-3p mimic group j. Statistical analysis of alizarin red s staining results (n = 5) k. Alkaline phosphatase staining after 7 days of osteogenic differentiation in the miR-130b-3p mimic group l. Statistical analysis of alkaline phosphatase staining results (n = 5) *:p<0.05