Figure 5

Analysis of usherin and Whrnb expression in retinal sections of wild-type, adgrv1^rmc22, and adgrv1^Δexon40-42 zebrafish

Retinal cryosections of wild-type, mutant adgrv1^rmc22, and adgrv1^Δexon40-42 zebrafish larvae (5 dpf) labeled with antibodies directed against Whrnb (red) (A) or usherin (red) (B) and centrin (green). Nuclei are counterstained with DAPI (blue). (A) Both in wild-type and in adgrv1^Δexon40-42 zebrafish larvae, Whrnb was present at the photoreceptor periciliary region in close proximity to centrin. Quantification revealed that the Whrnb signal intensity in adgrv1^Δexon40-42 retinal sections is similar to that in wild-type sections, whereas a significant reduction of Whrnb signal was observed in retinae of adgrv1^rmc22 larvae. Magnified images are shown at the right side. Bar graphs represent the mean fluorescence intensity of anti-Whrnb staining of all photoreceptors of a single, central section of one larval zebrafish eye (n = 14 eyes). (B) Both in wild-type and adgrv1^Δexon40-42 zebrafish larvae, usherin was present at the photoreceptor periciliary region in close proximity to centrin. Quantification reveals that the usherin signal intensity in retinal sections of adgrv1^Δexon40-42 larvae is similar to that in sections of wild types, whereas a significant reduction of usherin signal was observed in retinae of adgrv1^rmc22 larvae. Bar graphs represent the mean fluorescence intensity of anti-usherin staining of all photoreceptors of a single, central section of one larval zebrafish eye, with mean ± SD (n = 14 eyes). Data were analyzed using one-way ANOVA followed by Tukey’s multiple comparison test (∗∗p < 0.01; ∗∗∗∗p < 0.0001). Scale bars, 10 μm.