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In silico modeling of the ADGRV1 protein domain architecture after the excision of ADGRV1 exon 9 and exons 40–42 (A) Schematic representation of the protein domain structure of the large isoform (isoform B) of human and zebrafish ADGRV1, based on 2D-SMART protein predictions. Regions encoded by exon 9 and exons 40–42 are highlighted with dashed boxes. (B) In silico 3D modeling of human ADGRV1, based on AlphaFold2. The Calxβ domains encoded by ADGRV1 exon 9 are depicted in cyan. (C) In silico 3D modeling of the protein domains encoded by exons 6–12 in man and zebrafish. The part encoded by exon 9 is depicted in cyan (human ADGRV1) or green (zebrafish Adgrv1). Removal of exon 9 is predicted to result in a discontinuity in the Calxβ domain structure. (D) In silico 3D modeling of human ADGRV1, based on AlphaFold2 predictions. The parts of Calxβ domains encoded by ADGRV1 exons 40–42 are depicted in cyan. (E) In silico 3D modeling of the protein domains encoded by exons 35–45 in man and zebrafish. The part encoded by exons 40–42 is depicted in cyan (human ADGRV1) or green (zebrafish Adgrv1). Removal of exons 40–42 results in the production of a single-hybrid Calxβ domain, with a 3D structure highly similar to native Calxβ domains. (F) Structural comparison of native human and zebrafish Calxβ domains 21, along with the hybrid Calxβ domain resulting from the removal of exons 40–42 in both man and zebrafish. The superimposed image shows that the 3D structures of the hybrid human (blue) and zebrafish (magenta) Calxβ domains closely resemble the conformations of the native human Calxβ domain 21 (blue–cyan) and zebrafish Calxβ domain 21 (magenta–green).
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