Figure 6

Analysis of rhodopsin localization in retinal sections of wild-type, adgrv1^rmc22, and adgrv1^Δexon40-42 zebrafish

(A) Schematic representation of a photoreceptor with rhodopsin localization in the outer segments (OS) and aberrant rhodopsin localization in the photoreceptor cell body as observed in adgrv1^rmc22 larvae. (B) Retinal cryosections of wild-type, adgrv1^rmc22, and adgrv1^Δexon40-42 zebrafish larvae (6 dpf) labeled with antibodies directed against rhodopsin (green). Nuclei are counterstained with DAPI (blue). As observed in the retinas of wild-type and adgrv1^Δexon40-42 zebrafish larvae, rhodopsin predominantly localized to the photoreceptor outer segments. Photoreceptor cells with aberrant localization of rhodopsin were regularly observed in adgrv1^rmc22 larvae (indicated with the white arrows). Scale bars, 10 μm, OS, outer segments; ONL, outer nuclear layer; INL, inner nuclear layer. (C) Total number of cells with aberrant rhodopsin localization per retinal section were plotted, with mean ± SD (n = 41 wild types, n = 42 adgrv1^Δexon40-42, and n = 40 adgrv1^rmc22 larvae). Data were analyzed using one-way ANOVA followed by Tukey’s multiple comparison test (∗∗∗∗p < 0.0001).