Figure 1.

Characterization of gelatin microspheres: (a) field emission scanning electron microscopic (FESEM) image cross-linked gelatin microspheres (cG) using 50 mM DMTMM, scale bar = 5 μm, (b) size distribution of cG by laser diffraction, (c) surface charge on gelatin microspheres before crosslinking (G) and after crosslinking (cG) using zetasizer (N = 3), Error bars represent standard deviations, (d) Young’s modulus estimation of gelatin hydrogels cross-linked with 10, 50, and 100 mM DMTMM, termed as cG1, cG2, cG2 respectively, N = 3, Error bars represent standard deviations, (e) design of microfluidics chip, consists of deformability array with gap sizing of 8 μm, and capture array with v-cup sizing of 13 μm, (f) representation of deformable gelatin microspheres (>8 μm) captured in the deformable array, scale bar = 10 μm, and (g) representation of aggregated spheres/smaller spheres/non-deformable spheres captured in V-cups, scale bar = 10 μm. Graphs are plotted in box and whiskers format with max and min value showing all data points.