Fig. 8

Lethality and neurotoxicity evaluation of A-pure and B-pure colistin after melatonin, EGCG or curcumin pre-exposure. 2 hpf zebrafish embryos were exposed to melatonin, EGCG or curcumin for 4 h. Afterwards, solutions were changed to 5 µM of A-pure colistin or 10 µM of B-pure colistin until 72 hpf where the lethality was recorded, and the TER assay was performed. A) Percentage of accumulated lethality after A-pure colistin exposure. B) Percentage of accumulated lethality after B-pure colistin exposure. C) TER assay after A-pure colistin exposure. D) TER assay after B-pure colistin exposure. In all cases results are expressed as mean ± SEM of 3 independent experiments with 10 or 6 embryos per group for lethality or TER assay, respectively. Statistical analysis was performed using One-way ANOVA with Bonferroni’s pos-hoc test. * Indicates significant differences with the control. # indicates significant differences with the colistin group. In all cases p < 0.05 was considered for statistical differences.