Figure 3

Wild-type circulating cells partially restore the regeneration potential in terfa morphants. (A) Schematic representation of parabiosis experiment using Tg(mpeg1:gal4/UAS:NTR-mcherry) larvae previously injected with terfa MO or ctrl MO and wild-type (WT) larvae. Conjoined embryos were generated at the shied stage. Macrophages in the morphant partner were depleted by injection of 10 mM metronidazole at 48hpf. The morphants' caudal fin folds were amputated at 72 hpf. Then, at 72 hpA, regeneration was analyzed by measuring the length of the regenerated caudal fin fold. (B) Representative images of conjoined embryos at 72 hpA. The middle panels show a zoom of the cut caudal fins. The right panels show a zoom of regenerated fins at 72 hpA. (C) Quantification of the regenerated caudal fin length at 72 hpA (data are the mean ± SEM, n = 11 Ctrl MO larvae, n = 3 terfa MO larvae).