FIGURE

Fig. 7

ID

ZDB-FIG-250728-80

Publication

Gregory-Evans et al., 2025 - Mutation of beta-tubulin 4B gene (TUBB4B) causes autosomal dominant retinitis pigmentosa with sensorineural hearing loss in a multigenerational family

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

This figure shows confirmation of tubb4b morpholino knockdown. (A) Schematic representation of MO-1 splice-blocking that removes exon 2 (Ex 2) in the mutant (mt) mRNA transcript. FP refers to the forward primer; RP refers to the reverse primer; utr refers to the untranslated region. (B) Representative RT-PCR gel confirming reduction in size of tubb4b amplicon. MO1 refers to splice-blocking morpholino; SM refers to size marker; WT refers to the wild-type. (C) Representative image of a western blot of tubb4b protein in zebrafish injected with MO2 translation-blocking morpholino. The gel loading control is gapdh. MW refers to molecular weight size marker and WT refers to the wild-type. (D) Representative RT-PCR gels showing levels of rhodopsin (rho) and tubb6 transcripts with MO2 translation-blocking morpholino knockdown relative to gapdh expression.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.