FIGURE

Fig. 4

ID

ZDB-FIG-250722-46

Publication

Sweeney et al., 2025 - Creb3l3 deficiency promotes intestinal lipid accumulation and alters ApoB-containing lipoprotein kinetics

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Fig. 4

creb3l3 target genes are not induced in creb3l3 mutant intestines following a high-fat meal. A: creb3l3 target genes are clustered together on zebrafish Chr 16 and Chr 19. B: HFM-induced apoea expression in creb3l3a^c802/+;creb3l3b^c803/+ animals, but not in double mutants. Similarly, apoeb (C) and apoa4a (D) expression increases in creb3l3a^c802/+;creb3l3b^c803/+ animals after an HFM, but this response is attenuated in creb3l3a^c802/c802;creb3l3b^c803/c803 animals. E: apobb.1 expression is not significantly changed between creb3l3 double heterozygotes and double homozygotes after an HFM. A failure to induce mRNA expression of apoa4b.1 (F), apoa4b.2 (G), and apoa4b.3 (H) was also noted in creb3l3 mutants after an HFM. Relative mRNA expression of each gene was normalized to 18S RNA. N = 3 clutches; n = 6–14 individuals per clutch per condition. One way ANOVA with Tukey’s multiple comparisons. ∗∗∗∗P < 0.0001, ∗∗∗P < 0.001, ∗∗P < 0.01, and ∗P < 0.05. Creb3l3, cAMP-responsive element-binding protein 3–like 3; HFM, high-fat meal.

Expression Data

Genes:	apoa4a apoa4b.1 apoa4b.2 apoa4b.3 apobb.1 apoea apoeb
Fish:	creb3l3a^c802/+; creb3l3b^c803/+ (AB) creb3l3a^c802/c802; creb3l3b^c803/c803 (AB)
Conditions:	high fat fasting
Anatomical Term:	intestine
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	creb3l3a^c802/+; creb3l3b^c803/+ (AB) creb3l3a^c802/c802; creb3l3b^c803/c803 (AB)
Condition:	high fat
Observed In:	intestine
Stage:	Day 6

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Lipid Res.