Fig. 7

The loss of Creb3l3 may affect lipid secretion from the enterocyte. A: Fluorescent signal of BODIPY-C12 within intestinal homogenate over 24 h post gavage shows no significant difference between apoC2^sd38 animals and apoC2^sd38/+ control animals. B: 24-h postprandial trend of BODIPY-FL C12 fluorescent signal in blood plasma. Fluorescent signal in apoC2^sd38 animals remains elevated from 4 h to 24 h post gavage compared to apoC2^sd38/+ animals. C: Fluorescent signal within intestinal homogenate over 24 h post gavage. Creb3l3 mutants show slightly less total fluorescence over time, yet there is no significant difference between creb3l3 double homozygous mutants and creb3l3 double heterozygote control animals. D: Blood plasma BODIPY-FL C12 fluorescent signal is reduced in creb3l3 double homozygous mutants and significantly different at 6 h post gavage. For each experiment, the mean fluorescent signal at each time point was divided by the mean fluorescent value of the control (heterozygotes) at t = 0 to calculate the fold change between the individual genotypes. The average fold change for each experiment was plotted. n = 3 individual fish of each genotype at each time point for each experiment; three independent experiments for each time course. ∗ = P < 0.05, Two-way ANOVA with multiple comparisons. Creb3l3, cAMP-responsive element-binding protein 3–like 3.