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Fig. 1

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ZDB-FIG-250716-87

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Sewgoolam et al., 2025 - Genome-wide antibiotic-CRISPRi profiling identifies LiaR activation as a strategy to resensitize fluoroquinolone-resistant Streptococcus pneumoniae

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Fig. 1

CRISPRi-seq identifies pneumococcal genes influencing fluoroquinolone susceptibility at a genome-wide level.

A Schematic overview of the chemogenomic screen workflow. The S. pneumoniae CRISPRi library was treated with a sub-lethal dose of each antibiotic (AB) in the absence or presence of IPTG to induce dCas9. The results of the pooled CRISPRi-seq screen were then validated by testing individual mutants. B Heatmap depicting all the sgRNAs (representing operons that have been transcriptionally repressed) that showed a significant differential fitness effect upon antibiotic treatment shared between all three fluoroquinolones as compared to no antibiotic treatment (|Δlog₂FC| > 1, P_adj < 0.05; DESeq2³² differential enrichment analysis using a negative binomial generalized linear model with two-tailed P-values adjusted for false discovery rate (FDR)). Source data available in Supplementary Data 1.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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