FIGURE

Fig. 2

ID

ZDB-FIG-250716-88

Publication

Sewgoolam et al., 2025 - Genome-wide antibiotic-CRISPRi profiling identifies LiaR activation as a strategy to resensitize fluoroquinolone-resistant Streptococcus pneumoniae

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Fig. 2

The ssDNA repair complex RecJFOR is crucial for pneumococcal survival during fluoroquinolone treatment.

A At DNA gaps, the ssDNA-specific exonuclease RecJ enlarges the ssDNA region. RecF is a recombination mediator protein and works in complex with RecOR to facilitate the binding of RecA onto the ssDNA. The recombinase RecA, then, promotes homology search, homologous pairing, and strand exchange. Figure adapted from ref. ³⁸. Depletion strains were constructed for (B) recJ and CrecF, and the mutants were treated with the sub-lethal concentration of LEV used in the screens. Deletion of recF or recJ resulted in increased sensitivity to LEV, and the phenotype was rescued upon complementation. D Deletion of efp resulted in a fitness advantage in the presence of a lethal concentration of LEV. Growth curve data represent the mean ± SEM of three biological replicates. E Blocking translation elongation using chloramphenicol (CHL) results in an antagonistic interaction with LEV. Source data are provided as a Source Data file.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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