Fig. 1

Fig. 1 CRISPRi-seq identifies pneumococcal genes influencing fluoroquinolone susceptibility at a genome-wide level.

A Schematic overview of the chemogenomic screen workflow. The S. pneumoniae CRISPRi library was treated with a sub-lethal dose of each antibiotic (AB) in the absence or presence of IPTG to induce dCas9. The results of the pooled CRISPRi-seq screen were then validated by testing individual mutants. B Heatmap depicting all the sgRNAs (representing operons that have been transcriptionally repressed) that showed a significant differential fitness effect upon antibiotic treatment shared between all three fluoroquinolones as compared to no antibiotic treatment (|Δlog₂FC| >  1, P_adj < 0.05; DESeq2³² differential enrichment analysis using a negative binomial generalized linear model with two-tailed P-values adjusted for false discovery rate (FDR)). Source data available in Supplementary Data 1.