Fig. 5

Reduced islet volume and decreased numbers of insulin-producing beta cells following exocrine damage. (a) 3D reconstruction of GFP⁺ beta cells and the islet volume measurement after 5 µmol/l NFP treatment. Scale bar, 25 µm. (b) Bar blots demonstrating the impact of NFP on the islet volume in Tg(ela3l:ntr;insulin:gfp) larvae (n=15 each). Data represent the mean values with bars indicating the range from the maximum to the minimum data points. Mann–Whitney U test was used for statistical differences between groups: **p<0.01. (c) Experimental schematic for imaging insulin-labelled Tg(ela3l:ntr;insulin:gfp) at 96 hpf after 6 and 12 h of NFP treatment followed by 12 h of medium without NFP (recovery time). (d, e) 3D reconstruction of insulin immunofluorescent staining and DAPI staining at 120 hpf in whole larvae following NFP treatment (n=5). Scale bar, 100 µm. Data represent the median values with bars indicating the range from the maximum to the minimum data points. Unpaired t test was used for statistical differences between groups:, ***p<0.001. Fig. 5c is created in BioRender. Faraj, N. (2025) https://BioRender.com/g20u364