FIGURE

Fig. 3

ID

ZDB-FIG-250714-99

Publication

Faraj et al., 2025 - Pancreatic exocrine damage induces beta cell stress in zebrafish larvae

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Exocrine damage induces ER stress in beta cells. (a) Schematic showing the ER stress reporter under the beta cell-specific insulin promoter; venus is fused with xbp1, which under normal conditions remains unspliced, resulting in only mTurquoise2⁺ nuclei. Upon ER stress, XBP1s is produced with Venus being in frame and causing stressed beta cells to display mTurquoise2⁺ nuclei and Venus⁺ cytosol in Tg(insulin:h2b-mturquoise.2-xbp1-venus;cryaa:mcherry) larvae, briefly Tg(insulin:xbp1v). Additionally, mcherry under the cryaa promoter is included to facilitate the selection of positive transient zebrafish. (b) The expression of mTurquoise2 and Venus in beta cells after exocrine damage stimulation by 5 µmol/l NFP. Scale bar, 50 μm or 10 µm (magnified image). (c) Quantification of the ratio of stress beta cells to the total number of beta cells after 6 and 12 h of NFP treatment (n=5 each). The representative images were used for quantification. Scale bar, 10 µm. Data represent the median values with bars indicating the range from the maximum to the minimum data points. Unpaired t test was used for statistical differences between groups. ***p<0.001. Part of Fig. 3a is created in BioRender. Faraj, N. (2025) https://BioRender.com/z06r241

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Diabetologia