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Fig. 6

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ZDB-FIG-250709-27

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Wieloch et al., 2025 - In vitro embryolethality testing during the peri-implantation stage using 3D mouse embryoids: comparison with 2D mouse cell cultures and the zebrafish embryo model

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Fig. 6

Metabolic activity of 3D embryoids (DIV4), 2D ESC-TSC-XENC and lethality of zebrafish embryos in the presence of penicillin-G, D-mannitol and artemisinin. (A, E) Viability of DIV4 embryoids exposed for 48 h to increasing concentrations of penicillin-G, D-mannitol and artemisinin by means of metabolic activity measured by CellTiterGlo-3D™ assay. (B, F) viability of ESC (yellow circles), TSC (blue squares) and XENC (magenta triangles) exposed for 48 h to increasing concentrations of penicillin-G, D-mannitol and artemisinin by means of metabolic activity measured by CellTiterGlo-3D™ assay. (C, D, G) lethality of zebrafish embryos exposed for 48 h to penicillin-G, D-mannitol and artemisinin, assessed according to the criteria described in TG 236 [29]. Results representing the mean ± SEM of at least three independent experiments. Statistical analysis of mouse embryoids and zebrafish embryos results was performed with one-way ANOVA followed by Dunnett post hoc comparison test, while for 2D cell cultures a two-way ANOVA followed by Tukey’s post hoc comparison test was performed to allow for evaluation of significant differences compared to the respective solvent control (*) and significant differences between cell lines: (a) ESC vs. XENC, (b) XENC vs. TSC (c) ESC vs. TSC. Significance threshold was established at p < 0.05 in all cases.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

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Reprinted from Reproductive toxicology (Elmsford, N.Y.), , Wieloch, J., Blanco, J., Zordick, C., Ohnesorge, N., Schneider, M.R., Barenys, M., Knöspel, F., In vitro embryolethality testing during the peri-implantation stage using 3D mouse embryoids: comparison with 2D mouse cell cultures and the zebrafish embryo model, 108941108941, Copyright (2025) with permission from Elsevier. Full text @ Reprod. Toxicol.