Figure 4

Histologic analysis of retinopathy and prophylactic treatment with TnP. Retinopathy was induced in 3 dpf larvae through exposure to E2 0.5x medium at 28 °C with 0.5 mM cobalt chloride (CoCl₂) for 72 h, and one group was prophylactically treated with 100 µM TnP for same period. Independent group of 3 dpf larvae was exposed to intense light at 8000 lux for 24 h (LIRD), and one group was prophylactically treated with 100 µM TnP for same period. Retinal layer thickness of CoCl₂-exposed zebrafish larvae (A) and zebrafish larvae subjected to LIRD (B). * p < 0.05 compared to control group. # p < 0.05 compared to disease group. Bars represent mean ± SEM. Dots represent individual data points. PRL: photoreceptor layer; ONL: outer nuclear layer; OPL: outer plexiform layer; RPE: retinal pigment epithelium; INL: inner nuclear layer; IPL: inner plexiform layer; GCL: ganglion cell layer.