Fig. 7

Roraa directly regulates core genes of the PPAR signaling pathway, influencing PPAR signaling activity. A–D Validation of changes in the expression of me1, cpt2, fads2, and fabp2 in WT and roraa mutants under LD conditions via real-time quantitative PCR. E–H Validation of changes in the expression of me1, cpt2, fads2, and fabp2 iin WT and roraa mutants under DD conditions via real-time quantitative PCR. The JTK cycle was used to determine whether gene expression was rhythmic, and differences in expression levels between the WT and mutant strains were analyzed at each time point. I–L Dual-luciferase reporter assays were used to detect the regulatory effects of Roraa on the activation of the me1, cpt2, fads2, and fabp2 promoters. Red represents mutants, whereas black represents WT; p values indicate results from JTK cycle rhythmicity analysis, with p < 0.05 indicating rhythmicity and p > 0.05 indicating a lack of rhythmicity. The method for difference analysis was Student's t test