Fig. 4

Transcriptional regulation of the clock gene per2 by Roraa in zebrafish. A Analysis of the cloned promoter sequence elements on the per2-luc plasmid, with a sequence length of 595 bp that includes one RORE element. A schematic diagram showing the deletion of the RORE in the per2 promoter sequence. B The gradient activation effect of Roraa on per2 expression, with activation increasing with increasing concentrations of Roraa at 100 ng, 200 ng, and 400 ng. C The activation effect of Roraa on per2 disappeared after the deletion of the RORE element, indicating the key role of the RORE element in Roraa's regulation of per2 expression. D Comparison of the relative expression levels of per2 in zebrafish treated with SR3335 and SR1078. “ + ” indicates cells transfected with the specific plasmid, whereas “-” indicates the control group without plasmid transfection. The method for difference analysis was Student's t test; ns indicates p > 0.05, * indicates p < 0.05, and *** indicates p < 0.001