Fig. 6

Activity changes in the MnR under social conflict (A) Expressions of GCaMP6f in 5-HT neurons of the MnR. (Upper) GCaMP6f expression (green) was limited near the midline of the MnR. White dotted line indicates the trace of implanted fiber tip. Scale bar, 100 μm. (Lower) Signals of GCaMP6f (green) colocalized in 5-HT antibody signals (magenta) in the MnR. Scale bars, 20 μm. (B) Schematic illustration of experimental schedule and treatment for Cont and cCKD mice. MnR activity was recorded in the home cage and then, immediately after, recorded under the condition as an intruder, from both Cont and cCKD mice. (C and D) The total time spent for offensive behaviors by the intruder Cont (n = 6) or cCKD (n = 4) to the resident B6 WT mice (p = 0.0312) (C) and defensive behaviors of the Cont or cCKD from the resident mice (p > 0.05) (D). (E) Typical traces of Z scored calcium peaks during the first 1 min recording at home cage and as an intruder from both Cont and cCKD mouse. Orange dots represent positive peaks that were over the threshold. (F and G) Change in the total number of GCaMP6f signal peaks from home cage to those as an intruder in Cont (p = 0.0309) (F) and cCKD mouse (p = 0.0022) (G). (H) Normalized increase in the ratio of peaks of basal activity to activity as an intruder (p < 0.001). Dotted line represents 100%. Bars, mean. Unpaired t test was used for all statistical analyses except for (F) and (G). Paired t test was used for (F) and (G). Statistical significance was defined as ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001.