Fig. 7

The detection of pericardial edema inhibition in zebrafish through fluorescence microscopy, assessing the combined effect of iMDK and Lenvatinib. (A) Zebrafish fertilized for 3 days were assigned to treatments corresponding to the control group and the administration group (iMDK, Lenvatinib, and a combination of both), with all compounds administered at a concentration of 1 μM. Images were captured using a fluorescence microscope, where green fluorescence identified the zebrafish blood vessels. White arrows indicate the site of pericardial edema, while the yellow dashed lines represent the SIVs. (B) Quantification of pericardial oedema area with ImageJ 2 software in zebrafish after treatment with iMDK, Lenvatinib, and a combination of iMDK and Lenvatinib. The area of pericardial oedema was defined as 100% for zebrafish in the blank group. (C) Quantification of SIV area with ImageJ 2 software in zebrafish after treatment with iMDK, Lenvatinib, and a combination of both iMDK and Lenvatinib. The SIV area was defined as 100% for zebrafish in the blank group. Compare the significance of the difference between each group and the blank group using Student's t-test. Probability values p < .05 indicate statistical differences, with statistical significance indicated by “*” for p < .05 and “**” for p < .01. Six individuals were included in each experimental group (n = 6). All these experiments were conducted three times in a double-blind manner, and the quantification results are presented as the mean ± SD.