Fig. 5

The detection of the inhibitory effect of the combination of iMDK and Lenvatinib on angiogenesis in zebrafish using fluorescence microscopy. (A) Zebrafish fertilized for three days were subjected to treatment in accordance with the control group and the administration groups, which included iMDK, Lenvatinib, and a combination of iMDK and Lenvatinib, with all inhibitor concentrations maintained at 1 μM. Images were obtained using a fluorescence microscope, where green fluorescence represented the zebrafish vasculature, while white arrows highlighted the intact ISVs. (B) Quantification of the numbers of ISVs in zebrafish to assess the inhibitory effect of iMDK, Lenvatinib, and their combination on these vessels. Compare the significance of the difference between each group and the blank group using Student's t-test. Probability values p < .05 denote statistical differences, with statistical significance marked by “*” for p < .05 and “**” for p < .01. Six individuals were included in each experimental group (n = 6). All these experiments were conducted three times in a double-blind manner, and the quantification results are presented as the mean ± SD.