Fig. 3.

Mutation influences the transport of mitochondria to the dendrites. The cultured brain neurons of wt and tomm70 mutant female fish were stained with DAPI (representing nucleus; blue), anti-β-Tubulin antibody (a neuronal marker; green), anti-Tomm70 antibody (red) and anti-Cytochrome c antibody (a conserved mitochondrial marker; cyan). The brightness of images was corrected using ImageJ. (A,B) Representative pictures of multi-polar neuronal staining of wt (A) and mutant (B) fish with anti-β-Tubulin, anti-Tomm70 and anti-Cytochrome c antibodies. The white-line box in the wide field column highlights the specific region magnified in the adjacent close-up column. White arrows in the mutants for β-Tubulin, Tomm70 and Cytochrome c staining indicate identical locations, underscoring the absence of Tomm70 and presence of Cytochrome c in all the neurites. Scale bars: 30 µm. (C) Quantification of the percentage of neuronal staining showing a signal for Tomm70 in neurites in wt and mutants. (D) Quantification of the percentage of neuronal staining showing a signal for Cytochrome c in neurites in wt and mutants. N=9 (wt) and 7 (−/−) for Tomm70, and N=4 (wt) and 3 (−/−) for Cytochrome c; n=29 (wt) and 32 (−/−) for Tomm70, and n=15 (wt) and 20 (−/−) for Cytochrome c. Error bar represents 95% c.i. Statistical significance was tested using Fisher's permutation test. **P<0.01; ns, non-significant.