Fig 9

nmd5∆/∆ has fully restored virulence in fish with a reduced immune response. (A) Kaplan-Meier survival curve of dexamethasone-treated hindbrain-injected fish with PBS (n = 28), SN250 (n = 41), nmd5∆/∆ (n = 43), or nmd5∆/∆+NMD5 (n = 35), or DMSO fish injected with PBS (n = 28), SN250 (n = 36), nmd5∆/∆ (n = 43), or nmd5∆/∆+NMD5 (n = 43). Data pooled from three independent experiments. (B) Kaplan-Meier survival curve of standard morphant fish injected with PBS (n = 22), SN250 (n = 46), nmd5∆/∆ (n = 39), or nmd5∆/∆+NMD5 (n = 35), and p47 morphant fish injected with PBS (n = 20), SN250 (n = 59), nmd5∆/∆ (n = 26), or nmd5∆/∆+NMD5 (n = 34). Data pooled from four independent experiments. (C) Kaplan-Meier survival curve of PBS (n = 34), SN250 (n = 37), or nmd5∆/∆ (n = 34) yolk-injected fish. Data pooled from two independent experiments. Statistics were performed as described in detail in Materials and Methods. Pairwise comparisons that are shown by arcs are confirmatory based on previous experiments; those shown by brackets are exploratory and adjusted for multiple comparisons by Bonferroni correction. Square brackets show if there is an effect of an immune perturbation on survival; curved brackets show if there is an effect of genotype on survival in the context of an immune perturbation. n.s., P_adj > 0.05; *P_adj < 0.05; **P_adj < 0.01; ***P_adj < 0.001.