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Hypovirulent C. albicans mutants elicit a reduced proinflammatory expression at 24 hpi. Expression of cxcl8b (A), tnfa (B), or il1b (C) by qPCR analysis of fish infected with WT (SN250), mutant (nmd5∆/∆, brg1∆/∆, or pep8∆/∆), or complemented (nmd5∆/∆+NMD5, brg1∆/∆+BRG1, or pep8∆/∆+PEP8) C. albicans at 24 hpi. Each point represents a pool of at least five larvae, and data were pooled from three (NMD5) or four (BRG1 and PEP8) independent experiments. Gene expression was normalized to gapdh, and induction was determined relative to PBS mock-infected larvae. Significance was determined by one-way ANOVA with Dunnett’s multiple comparisons tests.
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