Fig 3

Complementation restores virulence to hypovirulent C. albicans mutants. Kaplan-Meier survival curves show restoration of virulence with complementation. All data in survival curves are pooled from two experiments unless otherwise noted. (A) Fish injected with SN250 (WT, n = 37), brg1∆/∆ (n = 37), brg1∆/∆+BRG1 (n = 45), PBS (mock, n = 20). Complementation of brg1∆/∆ restores some virulence. (B) Fish injected with SN250 (WT, n = 49), pep8∆/∆ (n = 75), pep8∆/∆+PEP8 (n = 57), or PBS (mock, n = 30). Complementation of pep8∆/∆ restores some virulence (data pooled from three experiments). (C) Fish injected with PBS (mock, n = 20), SN250 (WT, n = 37), nmd5∆/∆ (n = 44), nmd5∆/∆+NMD5 (n = 41). Complementation significantly increases virulence of nmd5∆/∆. (D) Fish injected with SN250 (WT, n = 45), rim101∆/∆ (n = 43), rim101∆/∆+RIM101 (n = 41), or mock-infected fish (PBS, n = 20). Complementation of rim101∆/∆ restores virulence (data pooled from three independent experiments). (E) Fish injected with SN250 (WT, n = 35), cek1∆/∆ (n = 41), cek1∆/∆+CEK1 (n = 45), or mock-infected fish (PBS, n = 19). Complementation of cek1∆/∆ restores virulence. (F) Fish injected with PBS (mock, n = 21), SN250 (WT, n = 34), apm1∆/∆ (n = 39), or apm1∆/∆+APM1 (n = 28). Complementation significantly increases virulence of apm1∆/∆. (G) Fish injected with PBS (mock, n = 18), SN250 (WT, n = 40), mad2∆/∆ (n = 43), or mad2∆/∆+MAD2 (n = 39). Complementation significantly increases virulence of mad2∆/∆. *P_adj < 0.05, **P_adj < 0.01, ***P_adj< 0.001, ****P_adj< 0.0001.