High-throughput virulence screening. Average survival of fish infected with individual mutant C. albicans strains (n ≈ 50 fish per mutant strain). Mock-infected (PBS) and NRG1OEX-infected fish were included as controls. The average survival of the WT SN250 strain is shown by the black line, while differential survival was measured by z-score (based on the standard deviation of % survival in over 20 experiments with SN250 control infections). Gray lines show a z-score = 1, blue lines show a z-score = 2, and red, a z-score = 3. Strains in the red panel were previously seen to have a morphogenesis defect on Spider agar, while those in the blue panel showed a defect in pooled virulence tests, and those in the green panel code for predicted secreted proteins. Mutant strains that had a z-score of over 3 were passed to the next phase of screening, shown as squares. Strains in which both independent mutants showed hypovirulence had their genotypes PCR-confirmed and in which complementation restored virulence are shown as filled squares and were passed to the imaging phase of screening. Those that did not pass secondary screening are shown as empty squares. Complete data are found in Table S1.
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