FIGURE

Fig. 7

ID

ZDB-FIG-250505-119

Publication

Chen et al., 2025 - G protein-coupled receptor GPR182 negatively regulates sprouting angiogenesis via modulating CXCL12-CXCR4 axis signaling

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

GPR182 modulates CXCR4 signaling through internalizing CXCL12. (A and D) FRET analyses via acceptor photobleaching assess the formation of CXCR4 homodimers (positive control) and CXCR4/GPR182 heterodimers. Representative images display CFP and YFP fluorescent signals before and after photobleaching. White rectangles indicate the regions of interest (ROI) for photobleaching; negative controls lack photobleaching. (B, C, E, and F) Relative intensities of CFP and YFP in the corresponding ROIs for panels A (B and C) and D (E and F). (G) Confocal microscopy images depict CXCL12 internalization mediated by GPR182. HEK293T cells expressing YFP-tagged GPR182 are incubated with or without CXCL12, and endocytosis is assessed using an anti-CXCL12 antibody. (H) Quantification of intracellular GPR182 localization as shown in panel G. (I) Intracellular colocalization of GPR182 and Rab5 upon CXCL12 addition. HEK293T cells expressing YFP-tagged GPR182 are incubated with or without CXCL12, and cells are stained with an anti-Rab5 antibody

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Angiogenesis