Fig. 7.

Induction of Gq activity upregulates dlx5a expression and downregulates nr2f5 expression. (A-H′) The expression patterns of dlx5a and nr2f5, both in magenta, are shown by two-color fluorescence in situ hybridization on 28 hpf embryos. Pharyngeal arches are labeled, with dlx2a in green. dlx5a and dlx2a (A,B,E,F) or nr2f5 and dlx2a (C,D,G,H) are shown overlaid. dlx5a (A′,B′,E′,F′) and nr2f5 (C′,D′,G′,H′) are also shown alone, with the border of the pharyngeal arches indicated by white dashed lines. (A,A′,C,C′) Expression in non-transgenic edn1^+/+ embryos. (E,E′,G,G′) Expression in hsp70l:Gq-Q209L;edn1^+/+ embryos. (B,B′,D,D′) Expression in non-transgenic edn1^−/− embryos. (F,F′,H,H′) Expression in hsp70l:Gq-Q209L;edn1^−/− embryos. All embryos were heat-shocked. Images are representative of at least five embryos. Scale bar: 50 μm. cf, choroid fissure; ov, otic vesicle.