FIGURE

Fig 6

ID

ZDB-FIG-250501-52

Publication

Zhou et al., 2025 - A sensitive soma-localized red fluorescent calcium indicator for in vivo imaging of neuronal populations at single-cell resolution

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Fig 6

Dual-color in vivo calcium imaging of NTS tuning during gastric distension.

(A) Schematics depicting NTS two-photon calcium imaging setup during gastric distension. (B) Representative two-photon NTS images showing the co-expressing of RiboL1-jGCaMP8s and SomaFRCaMPi with the shared ROIs identified from Cellpose segmentation. Scale bar, 50 μm. (C and D) Heat maps depicting time-resolved responses to a series of gastric distensions from NTS neurons co-expressing Ribo-GCaMP8s (C) and SomaFRCaMPi (D) from the image in (B) (329 neurons). (E) Scatter plot showing the peak ΔF/F of green (Ribo-GCaMP8s) and red (SomaFRCaMPi) calcium responses from NTS neurons co-expressing both indicators during gastric distension. (F–G) Similar to (E), but for noise evaluation (F) and peak Z-score analysis (G). n = 1,580 neurons from 2 mice. The grey diagonal dash represents a slope equal to 1, and the black dashed line denotes the linear fit of the data. The quantitative data presented in this figure can be found in S1 Data.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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