ZFIN Image: Zhou et al., 2025, Fig 6

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Figure Caption

Fig 6 Dual-color in vivo calcium imaging of NTS tuning during gastric distension.

(A) Schematics depicting NTS two-photon calcium imaging setup during gastric distension. (B) Representative two-photon NTS images showing the co-expressing of RiboL1-jGCaMP8s and SomaFRCaMPi with the shared ROIs identified from Cellpose segmentation. Scale bar, 50 μm. (C and D) Heat maps depicting time-resolved responses to a series of gastric distensions from NTS neurons co-expressing Ribo-GCaMP8s (C) and SomaFRCaMPi (D) from the image in (B) (329 neurons). (E) Scatter plot showing the peak ΔF/F of green (Ribo-GCaMP8s) and red (SomaFRCaMPi) calcium responses from NTS neurons co-expressing both indicators during gastric distension. (F–G) Similar to (E), but for noise evaluation (F) and peak Z-score analysis (G). n = 1,580 neurons from 2 mice. The grey diagonal dash represents a slope equal to 1, and the black dashed line denotes the linear fit of the data. The quantitative data presented in this figure can be found in S1 Data.

Acknowledgments

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