FIGURE

Fig. 3

ID

ZDB-FIG-250421-75

Publication

Huybrechts et al., 2025 - Loss of the Ubiquitin-Associated Domain of sqstm1/p62 in Zebrafish Causes a Phenotype Resembling Paget's Disease of Bone

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Fig. 3

Vertebral columns of sqstm1tmΔUBA zebrafish are characterized by structural defects and ectopic mineralization. a Representative µCT images at 6 and 12 mpf. Vertebral malformations, including fused vertebrae, are evident in carriers of one or two mutant sqstm1 alleles, mainly in the precaudal to caudal vertebral junction and/or the tail region (red squares). b Close-up of the red squares shown in (a), labeled by 1–3. c Quantification of animals with certain number of vertebral defects. For 6 mpf, n = 4 (WT) or 6 (HET/HOM) zebrafish/genotype and for 12 mpf, n = 10 zebrafish/genotype. d Alizarin Red staining of a wild-type and homozygous zebrafish, including a magnification box. Ectopic mineralization and osteophytes affecting the whole vertebral column are shown by green arrows. WT wild type (sqstm1+/+), HET heterozygous mutant (sqstm1tmΔUBA/+), HOM homozygous mutant (sqstm1.tmΔUBA/tmΔUBA)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sqstm1^{zf4282/zf4282} (AB) sqstm1^zf4282/+ (AB)
Observed In:	bone element vertebra vertebral column vertebral column bone mineralization
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Calcif. Tissue Int.