FIGURE

Fig. 1

ID

ZDB-FIG-250421-73

Publication

Huybrechts et al., 2025 - Loss of the Ubiquitin-Associated Domain of sqstm1/p62 in Zebrafish Causes a Phenotype Resembling Paget's Disease of Bone

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Fig. 1

Generation of the sqstm1tmΔUBA zebrafish model. a cDNA sequence of the targeted region in sqstm1 for wild-type (sqstm1+/+) and homozygous mutant (sqstm1tmΔUBA/tmΔUBA) zebrafish, demonstrating the presence of the 14 base pair insertion (red). The protospacer adjacent motif (PAM) sequence and the actual upstream cleavage site (^) are marked using a line. b Schematic representation of the wild-type p62 protein domains (PB1, Phox, and Bem1; ZZ, zinc finger; UBA, ubiquitin-associated) and the effect of genome editing on the level of the C-terminal UBA domain, creating the truncated p62.tmΔUBA protein. c T-Coffee alignment of the wild-type UBA domain and the truncated UBA (tmΔUBA) domain shows conservation (cons) of the amino acid characteristics, which is indicated with following symbols/colors: (*) positions with an identical residue (red); (:) strongly similar properties (orange); and (.) weakly similar properties (yellow). Blank positions have strong dissimilarities (green)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Calcif. Tissue Int.