ZFIN Image: Huybrechts et al., 2025, Fig. 1

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Figure Caption

Fig. 1 Generation of the sqstm1tmΔUBA zebrafish model. a cDNA sequence of the targeted region in sqstm1 for wild-type (sqstm1+/+) and homozygous mutant (sqstm1tmΔUBA/tmΔUBA) zebrafish, demonstrating the presence of the 14 base pair insertion (red). The protospacer adjacent motif (PAM) sequence and the actual upstream cleavage site (^) are marked using a line. b Schematic representation of the wild-type p62 protein domains (PB1, Phox, and Bem1; ZZ, zinc finger; UBA, ubiquitin-associated) and the effect of genome editing on the level of the C-terminal UBA domain, creating the truncated p62.tmΔUBA protein. c T-Coffee alignment of the wild-type UBA domain and the truncated UBA (tmΔUBA) domain shows conservation (cons) of the amino acid characteristics, which is indicated with following symbols/colors: (*) positions with an identical residue (red); (:) strongly similar properties (orange); and (.) weakly similar properties (yellow). Blank positions have strong dissimilarities (green)

Acknowledgments

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