Fig. 1
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- ZDB-FIG-250415-24
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- Gupta et al., 2025 - The transmembrane glycoprotein Gpnmb is required for the immune and fibrotic responses during zebrafish heart regeneration
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gpnmb is upregulated in regenerating coronary endothelial cells and macrophages after cardiac cryoinjury in zebrafish. A. Experimental plan for the bulk RNA-seq analysis on sorted regenerating (-0.8 flt1:RFP+/cxcr4a:YFP+) and non-regenerating (-0.8 flt1:RFP+/cxcr4a:YFP?) coronary endothelial cells from Tg(-0.8flt1:RFP); TgBAC(cxcr4a:YFP) whole ventricles at 7 dpci. B. Heatmap showing endothelial marker genes (kdrl, cdh5, crcr4a, esm1, and apln) and candidate genes (gpnmb and plxdc2) in the regenerating and non-regenerating coronary endothelial populations. C. RT-qPCR analysis of gpnmb mRNA levels at 7 dpci in the injured tissue relative to sham-operated hearts; Ct values listed in Supplementary Table S1. D-E. Reanalysis of the published single-cell RNA sequencing (scRNA-seq) dataset GSE159032 (Hu et al., 2022); UMAP representation of the different macrophage clusters highlighting 1) their distribution at several time-points (D), 2) their identity (D?), and 3) gpnmb expression (D??); dot plots of gpnmb expression in macrophages in the adult zebrafish heart (E). F-G. in situ hybridization chain reaction (HCR) for gpnmb expression and immunostaining for Cav1 (F) and L-plastin (G) on sections of uninjured and 96 hpci ventricles. Scale bars: 100 ?m. |
Reprinted from Developmental Biology, , Gupta, S., Bajwa, G.K., El-Sammak, H., Mattonet, K., Günther, S., Looso, M., Stainier, D.Y.R., Marín-Juez, R., The transmembrane glycoprotein Gpnmb is required for the immune and fibrotic responses during zebrafish heart regeneration, , Copyright (2025) with permission from Elsevier. Full text @ Dev. Biol.