Fig. 3

gpnmb mutants exhibit reduced fibroblast activation after cardiac cryoinjury in zebrafish. A. GSE analysis plot of collagen formation, biosynthesis, and assembly from the 96 hpci gpnmb?/? vs gpnmb+/+ cryoinjured ventricles transcriptomic analysis. B. Heatmap showing differentially expressed genes of interest in the BZI tissue of gpnmb?/? and gpnmb+/+ cryoinjured ventricles at 96 hpci. C. RT-qPCR analysis of acta2 mRNA levels in the gpnmb?/? injured tissue normalized to gpnmb+/+ injured tissue at 7 dpci; Ct values listed in Supplementary Table S1. D. Immunostaining for ?SMA (myofibroblasts, white) with DAPI (DNA marker, blue) counterstaining on sections of gpnmb+/+ and gpnmb?/? cryoinjured ventricles at 7 dpci. E. ?SMA+ cell numbers in gpnmb+/+ and gpnmb?/? injured tissues at 7 dpci. F. AFOG staining on sections of gpnmb+/+ and gpnmb?/? cryoinjured ventricles at 30 dpci. G. AFOG staining on sections of gpnmb+/+ and gpnmb?/? cryoinjured ventricles at 90 dpci. H. Graphs showing the representation of groups (y-axis) of different scar area sizes (different colors) at 30 and 90 dpci for gpnmb+/+ and gpnmb?/? cryoinjured ventricles. Mean scar area sizes for gpnmb+/+ and gpnmb?/? at 30 dpci are 9.4 and 11.6 % respectively. Mean scar area sizes for gpnmb+/+ and gpnmb?/? at 90 dpci are 0.9 and 1.6 % respectively. White dotted lines delineate the injured area; white arrowheads point to ?SMA+ cells (E). Statistical tests: Non-parametric Mann-Whitney test (C), Student's t-test (E). Scale bars: 100 ?m (D) and 200 ?m (F and H). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)