Fig. 1

Fig. 1 gpnmb is upregulated in regenerating coronary endothelial cells and macrophages after cardiac cryoinjury in zebrafish. A. Experimental plan for the bulk RNA-seq analysis on sorted regenerating (-0.8 flt1:RFP+/cxcr4a:YFP+) and non-regenerating (-0.8 flt1:RFP+/cxcr4a:YFP?) coronary endothelial cells from Tg(-0.8flt1:RFP); TgBAC(cxcr4a:YFP) whole ventricles at 7 dpci. B. Heatmap showing endothelial marker genes (kdrl, cdh5, crcr4a, esm1, and apln) and candidate genes (gpnmb and plxdc2) in the regenerating and non-regenerating coronary endothelial populations. C. RT-qPCR analysis of gpnmb mRNA levels at 7 dpci in the injured tissue relative to sham-operated hearts; Ct values listed in Supplementary Table S1. D-E. Reanalysis of the published single-cell RNA sequencing (scRNA-seq) dataset GSE159032 (Hu et al., 2022); UMAP representation of the different macrophage clusters highlighting 1) their distribution at several time-points (D), 2) their identity (D?), and 3) gpnmb expression (D??); dot plots of gpnmb expression in macrophages in the adult zebrafish heart (E). F-G. in situ hybridization chain reaction (HCR) for gpnmb expression and immunostaining for Cav1 (F) and L-plastin (G) on sections of uninjured and 96 hpci ventricles. Scale bars: 100 ?m.