FIGURE

Figure 1

ID

ZDB-FIG-250328-5

Publication

Besio et al., 2025 - The administration of exogenous HSP47 as a collagen specific therapeutic approach

Other Figures

All Figure Page

Back to All Figure Page

Figure 1

Recombinant heat shock protein 47 (rHSP47) is taken up by human fibroblasts.

(A) The intracellular uptake and presence of rHSP47-GFP is shown by bright-field and immunofluorescence (n = 3, representative image is shown). (B and C) Representative immunofluorescence images of primary wild-type fibroblasts incubated with rHSP47-GFP and stained for the ER markers protein disulfide isomerase (PDI) and calnexin (n = 3), (D) with the cis-Golgi marker Golgi matrix protein 130 (GM130), and with markers of the secretory vesicles (n = 3) (E) coat protein complex 2 (COP2) (n = 3) and (F) kelch-like family member 12 (KLHL12) (n = 3). Colocalization of rHSP47-GFP with cis-Golgi and secretory vesicles, but not with ER, is indicated by the overlapping peaks in the graphs. Nuclei were counterstained with DAPI. Original magnification, ×63 (insets further magnified ×4.5).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ JCI Insight