Figure 1

Figure 1 Recombinant heat shock protein 47 (rHSP47) is taken up by human fibroblasts.

(A) The intracellular uptake and presence of rHSP47-GFP is shown by bright-field and immunofluorescence (n = 3, representative image is shown). (B and C) Representative immunofluorescence images of primary wild-type fibroblasts incubated with rHSP47-GFP and stained for the ER markers protein disulfide isomerase (PDI) and calnexin (n = 3), (D) with the cis-Golgi marker Golgi matrix protein 130 (GM130), and with markers of the secretory vesicles (n = 3) (E) coat protein complex 2 (COP2) (n = 3) and (F) kelch-like family member 12 (KLHL12) (n = 3). Colocalization of rHSP47-GFP with cis-Golgi and secretory vesicles, but not with ER, is indicated by the overlapping peaks in the graphs. Nuclei were counterstained with DAPI. Original magnification, ×63 (insets further magnified ×4.5).