Fig. 5
- ID
- ZDB-FIG-250324-40
- Publication
- Kobar et al., 2024 - tp53 R217H and R242H mutant zebrafish exhibit dysfunctional p53 hallmarks and recapitulate Li-Fraumeni syndrome phenotypes
- Other Figures
- All Figure Page
- Back to All Figure Page
LFS zebrafish mutants develop sarcoma-like tumors with a divergent anatomical distribution of tumor development and higher lifetime tumor incidence in the tp53 R242H/R242H mutants. (A) Live and fixed morphology of tp53 mutant tumors, indicated by white arrows, and histology panels represent H&E stained sections of the anatomical area of the tumor. (B) Kaplan-Meier analysis showing tumor-free survival and onset in the tp53 null (n = 52), R217H/R217H (n = 77), and R242H/R242H (n = 112). Stars show significance as tp53 null vs. R217H/R217H — p = 0.005, tp53 null vs. R242H/R242H — p = 0.08, R217H/R217H vs. R242H/R242H — p = 1 × 10−7. Percent tumor-free survival refers to the age a specific fish had died or was euthanized due to tumor development. Tumor development was not observed in the wildtype line during this time period. Scale bars for 20× are 200 μm. Scale bars for 100× and 200× are 100 μm. (C) Comparison of anatomical site of tumor development in tp53 null (n = 51), R217H/R217H (n = 49), and R242H/R242H (n = 117) that developed tumors. The infection category represents fish with severe infection phenotypes (dropsy and edema) and may indicate the presence of a leukemia/lymphoma. A “*” above the percentage indicates a significant difference compared to tp53 null (p < 0.05 — two-tailed two proportions Z-test). (D) Comparison of tumor fish sex across the tp53 null (n = 52), R217H/R217H (n = 46), and R242H/R242H (n = 111) mutants. A “*” above the percentage indicates a significant difference compared to tp53 null (p < 0.05 — two-tailed two proportions Z-test). |
Reprinted from Biochimica et biophysica acta. Molecular basis of disease, 1871, Kobar, K., Tuzi, L., Fiene, J.A., Burnley, E., Galpin, K.J.C., Midgen, C., Laverty, B., Subasri, V., Wen, T.T., Hirst, M., Moksa, M., Carles, A., Cao, Q., Shlien, A., Malkin, D., Prykhozhij, S.V., Berman, J.N., tp53 R217H and R242H mutant zebrafish exhibit dysfunctional p53 hallmarks and recapitulate Li-Fraumeni syndrome phenotypes, 167612167612, Copyright (2024) with permission from Elsevier. Full text @ BBA Molecular Basis of Disease