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ZDB-FIG-250324-36

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Kobar et al., 2024 - tp53 R217H and R242H mutant zebrafish exhibit dysfunctional p53 hallmarks and recapitulate Li-Fraumeni syndrome phenotypes

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Fig. 1

The fold change of expression of several p53 target genes following induction of the DNA damage pathway is decreased in tp53 mutants. Thirty 24 hpf embryos per sample were exposed to 0 or 30 Gy of IR and were collected at 30 hpf (6 hpi). Expression of puma was not detected in the 0 Gy tp53 null embryos and expression of tp53 was not detected in the 0 nor 30 Gy tp53 null groups. Data represents 2–4 technical replicates and 3–4 biological replicates. The fold change was normalized to the wildtype 0 Gy group for each gene. Outliers were identified and excluded using Grubbs' test with alpha <0.05. Statistical significance was determined by a two-way ANOVA and a Tukey's Honest Significant Difference test. Error bars represent standard deviation. The Y-axis is logarithmic scale 10.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

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Reprinted from Biochimica et biophysica acta. Molecular basis of disease, 1871, Kobar, K., Tuzi, L., Fiene, J.A., Burnley, E., Galpin, K.J.C., Midgen, C., Laverty, B., Subasri, V., Wen, T.T., Hirst, M., Moksa, M., Carles, A., Cao, Q., Shlien, A., Malkin, D., Prykhozhij, S.V., Berman, J.N., tp53 R217H and R242H mutant zebrafish exhibit dysfunctional p53 hallmarks and recapitulate Li-Fraumeni syndrome phenotypes, 167612167612, Copyright (2024) with permission from Elsevier. Full text @ BBA Molecular Basis of Disease