FIGURE

Fig 6

ID

ZDB-FIG-250321-14

Publication

Nonarath et al., 2025 - The USH3A causative gene clarin1 functions in Müller glia to maintain retinal photoreceptors

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Fig 6

Actin is disorganized in the outer retina of clrn1^-/- mutant zebrafish.

Transverse sections from 7 dpf wild-type and clrn1^-/- Tg(gfap:GFP) zebrafish (a) showing the ordered spacing and radial expansion of Müller glia of both wild-type and clrn1 mutant retina. (b) Higher magnification images of Müller glia apical projections of wild-type and clrn1^-/- zebrafish highlights disorganization of the mutant apical microvilli (c). Quantification of total Müller glia (MG) apical microvilli within a 40 µm² region of the central retina of wildtype and clrn1^-/- zebrafish (d). Müller glia (MG) apical microvilli length beyond the OLM (labeled with asterisk in b.) for wild-type and clrn1^-/- 7 dpf zebrafish. (***p<0.005,****p<0.001; Students T-test). Scale bars = (a) 100 µm; (b) 50 µm. Data points in (c,d) represent measurements from n = 10 zebrafish each condition. For (d) data points are an average per zebrafish in which 9-22 projections quantified. Schematic in (b) created in BioRender under License: https://BioRender.com/x12n534.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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