FIGURE

Figure S5.

ID

ZDB-FIG-250320-12

Publication

Doan et al., 2025 - Dock1 functions in Schwann cells to regulate development, maintenance, and repair

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Figure S5.

WISH reveals an interaction between Dock1 and Rac1 in the developing zebrafish PNS. (A–I) Lateral views of larvae showing mbp expression by WISH in control, sgRac1+Cas9, and sgRac3+Cas9 in WT dock1^+/+, HET dock1^stl145/+, and homozygous dock1^{stl145/stl145} MUT zebrafish. (A) The arrow points to strong mbp expression in the PLLn. (E and F) Arrowheads highlight decreased mbp expression in the PLLn. (J) Quantification of TEM of total axons in the PLLn. n = 6 fish per genotype and experimental condition. (K) The quantification of WISH was assessed by examining mbp expression along the entire PLLn in 4 dpf control, sgRac1+Cas9, and sgRac3+Cas9 in WT dock1^+/+, HET dock1^stl145/+, and homozygous dock1^{stl145/stl145} MUT zebrafish, compared between phenotypic scores and genotypes. (L and M) Gels and schematics of the PCR and restriction digest validation of sgRac1 and sgRac3, respectively. Here, and in all figures, the X symbol in the graph denotes a data point corresponding to the representative image shown. (A–I) Scale bar = 100 µm. Source data are available for this figure: SourceData FS5.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Biol.