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Tbk1 expression and survival in zebrafish larvae with CRISPR-induced deletion of the tbk1 gene. A Description of CRISPR-Cas9 tandem sgRNA targeting exons 1 and 2 or exons 4 and 5 of the tbk1 gene (top) and genotyping of injected embryos with gel electrophoresis of PCR products amplifying the deleted region. B tbk1 protein quantification by mass spectrometry in zebrafish larvae harboring mosaic tbk1 mutations or their noninjected controls (NIs) (unpaired t test, *p < 0.05). C RT‒qPCR measurement of tbk1 expression in zebrafish larvae with mosaic deletions of the tbk1 gene or NI controls between 6 hpf and 8 dpf (unpaired t test, ***p < 0.001). D Survival rate of zebrafish larvae injected with tbk1 or mismatch AMO (ns), CRISPR-Cas9 sgRNA targeting exons 1 and 2 or exons 4 and 5 of the tbk1 gene or NI controls (log-rank (Mantel‒Cox) test, *** p < 0.001).
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