FIGURE

Fig. 1

ID

ZDB-FIG-250315-49

Publication

Raas et al., 2025 - TBK1 is involved in programmed cell death and ALS-related pathways in novel zebrafish models

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Fig. 1

Motor behavior and motor neuron phenotype induced by tbk1 knockdown in zebrafish larvae.

A Western blot quantification (ratio to β-actin level) of tbk1 expression after antisense morpholino oligonucleotide (AMO)- and hTBK1 mRNA injections. B Traces representative of the touch-evoked escape response (TEER) in 48 hpf zebrafish injected with tbk1 or mismatch AMO and hTBK1 mRNA injections. C Quantitative analysis of the TEER velocity of 48 hpf zebrafish larvae injected with tbk1 or mismatch AMO and rescued with mRNA encoding human TBK1. One-way ANOVA with Tukey’s multiple comparisons test was used. *p < 0.05, **p < 0.01). D In vivo quantification of spinal motor neuron axonal length and number in 48 hpf transgenic Tg(mnx1:gal4/UAS:RFP) embryos injected with tbk1 or mismatch AMO.

Expression Data

Gene:	RFP
Antibody:	Ab4-tbk1
Fish:	AB AB + MO1-tbk1 ml2Tg; nkuasrfp1aTg (AB) ml2Tg; nkuasrfp1aTg + MO1-tbk1 (AB)
Knockdown Reagent:	MO1-tbk1
Anatomical Terms:	motor neuron whole organism
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-tbk1 ml2Tg; nkuasrfp1aTg + MO1-tbk1 (AB)
Knockdown Reagent:	MO1-tbk1
Observed In:	motor neuron motor neuron axon thigmotaxis whole organism
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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